av电影在线观看网址,色综合伊人99久久久久精品,农村妇女操逼内射视频,亚洲 欧美 日韩 在线三区,校花出白浆视频一区二区三区,91亚洲资源在线观看,中文字幕乱码不卡日韩一区二区,国产老熟女一区二区三区仙踪密林,比基尼美女扣逼被人操的视频

【文獻(xiàn)標(biāo)題】Environmental concentrations of antibiotics impair zebrafish gut health

【作者】Li Zhou，Samwel Mchele Limbu，Meilin Shen，et.al

【作者單位】華東師范大學(xué)（East China Normal University）

【文獻(xiàn)中引用產(chǎn)品】

魚丙二醛（MDA）ELISA試劑盒

【關(guān)鍵詞】Antibiotic，Intestinal microbiota，Gut health，Zebrafish

【DOI】doi.org/10.1016/j.envpol.2017.12.073

【影響因子(IF)】5.98

【出版期刊】《Environmental Pollution》

【產(chǎn)品原文引用】

 Biochemical assays

In order to minimize bias, each treatment contained 30 fish for biochemical analysis. The whole gut contents of each fish were weighed and homogenized with 9 vol (v/w) of 0.8% physiological saline. Then, the homogenate was centrifuged at 2500×g at 4℃ for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instructions. Malondialdehyde (MDA) was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Hengyuan, Shanghai). Superoxide dismutase (SOD), peroxidase (POD), reduced glutathione (GSH), acid phosphatase (ACP), and alkaline phosphatase (AKP) were measured using related commercial assay kits (Nanjing Jiancheng Institute,China). Results were recorded on a microplate reader (Epoch, BioTek, USA).

完整版PDF文獻(xiàn)請(qǐng)咨詢?cè)诰€客服或者聯(lián)XI我司業(yè)務(wù)員！

更多公司福利請(qǐng)關(guān)注“恒遠(yuǎn)生物”VX公眾號(hào)！